Bloodstream Infection Pathogen Targeted Next-generation Sequencing (tNGS) Kit CapitalBio

Features

(1) High sensitivity: The LOD can be as low as 100 copies/ml, enabling detection of pathogens with low abundance or at early stage of infection;

(2) Quick test: The turnaround time can be as fast as 13.5 h;

(3) Broad coverage: 95% of clinically prevalent pathogens are covered;

(4) Precision treatment: It can accurately identify important pathogens such as Coxsackievirus A6, A16, and A21, enabling precise medicine and epidemic early warning.

Applicable Populations and Scenarios

• Patients with suspected bloodstream infection: Those who have symptoms such as fever, chills, hypotension, and altered consciousness, and for whom the cause cannot be identified by traditional methods like blood culture.

• Patients with fever after invasive procedures: Those who have undergone invasive procedures such as central venous catheterization and surgery recently, and then develop symptoms suggestive of bloodstream infection like fever.

Order Information

Background Information

Bloodstream infection (BSI) is a life-threatening disease associated with high mortality rates. Delayed diagnosis and ineffective treatment may lead to septic shock, multiple organ failure, disseminated intravascular coagulation (DIC), and even death. Thus, rapid and precise etiological diagnosis is crucial for the optimal treatment and prognosis of BSI. Blood culture is the most widely used conventional etiological diagnostic method. However, the sensitivity of blood cultures is far from ideal. And it is also time-consuming, usually taking 3–7 days or longer time, which limits its clinical practice. In recent years, polymerase chain reaction (PCR) and metagenomic next-generation sequencing (mNGS) have been used for the etiological diagnosis of BSI. Regarding PCR, a presumptive diagnosis is necessary and misdiagnosis is common. For mNGS, the sensitivity and positive rate are still not ideal. As reported, targeted next-generation sequencing (tNGS) has a higher sensitivity, and shorter turnaround time than blood culture and blood mNGS. The application of blood tNGS for BS might improve clinical outcomes.